Macrophages play an essential role in wound healing due to their dynamic nature and functional plasticity, exhibiting highly heterogeneous morpho-kinetic behaviors depending on their activation states. However, quantitative analysis of macrophage behavior in in vivo settings remains limited, largely due to the complexity of their diverse morphologies and motility patterns over time. In this study, we present an analytic workflow to investigate macrophage dynamics in zebrafish. By computing a comprehensive set of morpho-kinetic features, we reveal the clear distinctions between M1 (pro-inflammatory) and M2 (anti-inflammatory) macrophages in terms of shape elongation, directional movement, and random-like motion. Based on these features, we classify macrophages in the transition period into M1-like and M2-like groups. We compare and analyze their behaviors, which allows us to estimate the timing of the phenotypic switch. In addition, we analyze the behavior of macrophages that do not express Tumor Necrosis Factor (TNF) and are not stimulated by wound signaling. In summary, this study provides a quantitative analysis of macrophage behavior during wound healing and suggests distinct behavioral landscapes across different macrophage activation states.